Molecular cloning and functional analysis of the Populus deltoides remorin gene PdREM.
Identifieur interne : 002582 ( Main/Exploration ); précédent : 002581; suivant : 002583Molecular cloning and functional analysis of the Populus deltoides remorin gene PdREM.
Auteurs : Shaofeng Li [République populaire de Chine] ; Xiaohua Su ; Bingyu Zhang ; Qinjun Huang ; Zanmin Hu ; Mengzhu LuSource :
- Tree physiology [ 1758-4469 ] ; 2013.
Descripteurs français
- KwdFr :
- Arbres (génétique), Arbres (métabolisme), Bois (croissance et développement), Clonage moléculaire (MeSH), Développement des plantes (génétique), Expression des gènes (MeSH), Gènes de plante (MeSH), Paroi cellulaire (métabolisme), Phosphoprotéines (génétique), Phosphoprotéines (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines de transport (génétique), Protéines de transport (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Structures de plante (croissance et développement), Structures de plante (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Bois, Populus, Structures de plante.
- génétique : Arbres, Développement des plantes, Phosphoprotéines, Populus, Protéines de transport, Protéines végétales.
- métabolisme : Arbres, Paroi cellulaire, Phosphoprotéines, Populus, Protéines de transport, Protéines végétales, Structures de plante.
- Clonage moléculaire, Expression des gènes, Gènes de plante, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Carrier Proteins (genetics), Carrier Proteins (metabolism), Cell Wall (metabolism), Cloning, Molecular (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Phosphoproteins (genetics), Phosphoproteins (metabolism), Plant Development (genetics), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Structures (growth & development), Plant Structures (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Trees (genetics), Trees (metabolism), Wood (growth & development).
- MESH :
- chemical , genetics : Carrier Proteins, Phosphoproteins, Plant Proteins.
- chemical , metabolism : Carrier Proteins, Phosphoproteins, Plant Proteins.
- genetics : Plant Development, Populus, Trees.
- growth & development : Plant Structures, Populus, Wood.
- metabolism : Cell Wall, Plant Structures, Populus, Trees.
- Cloning, Molecular, Gene Expression, Gene Expression Regulation, Plant, Genes, Plant, Plants, Genetically Modified.
Abstract
Remorins play vital roles in signal transduction, energy transformation, ion flow and transport in plants. Upregulation of remorins correlates with dehiscence and cell maturation; however, no studies have been performed to elucidate the function of remorins in tree species. In this study, a Populus deltoides (Marsh.) plasma membrane-binding protein remorin gene (PdREM) was cloned and characterized by investigating its expression pattern and creating transgenic hybrid poplar (P. davidiana Dode × P. bolleana Lauche) lines expressing sense or antisense PdREM. PdREM was specifically expressed in leaf buds, and immature and mature phloem in P. deltoides. Downregulation of PdREM increased plant height, stem diameter, number of leaves, size of the xylem and phloem zones and induced expression of cell wall biosynthesis- and microfibril angle (MFA)-related genes. Overexpression of PdREM retarded vegetative growth. PdREM may negatively regulate vascular growth by inhibiting secondary cell wall expansion in poplar. In addition, antisense PdREM transgenic poplar had a lower MFA, suggesting that PdREM might contribute to sheet strength and wood properties in poplar. This study sheds light on the molecular mechanism of PdREM in P. deltoides growth and development, and lays the foundation for future functional genomics research into wood formation and the genetic engineering of forest trees with improved wood quality traits.
DOI: 10.1093/treephys/tpt072
PubMed: 24072517
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Forestry Experiment Center of North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name></author><author><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name></author><author><name sortKey="Huang, Qinjun" sort="Huang, Qinjun" uniqKey="Huang Q" first="Qinjun" last="Huang">Qinjun Huang</name></author><author><name sortKey="Hu, Zanmin" sort="Hu, Zanmin" uniqKey="Hu Z" first="Zanmin" last="Hu">Zanmin Hu</name></author><author><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24072517</idno><idno type="pmid">24072517</idno><idno type="doi">10.1093/treephys/tpt072</idno><idno type="wicri:Area/Main/Corpus">002455</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002455</idno><idno type="wicri:Area/Main/Curation">002455</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002455</idno><idno type="wicri:Area/Main/Exploration">002455</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and functional analysis of the Populus deltoides remorin gene PdREM.</title><author><name sortKey="Li, Shaofeng" sort="Li, Shaofeng" uniqKey="Li S" first="Shaofeng" last="Li">Shaofeng Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Forestry Experiment Center of North China, Chinese Academy of Forestry, Beijing 100023, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Forestry Experiment Center of North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name></author><author><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name></author><author><name sortKey="Huang, Qinjun" sort="Huang, Qinjun" uniqKey="Huang Q" first="Qinjun" last="Huang">Qinjun Huang</name></author><author><name sortKey="Hu, Zanmin" sort="Hu, Zanmin" uniqKey="Hu Z" first="Zanmin" last="Hu">Zanmin Hu</name></author><author><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carrier Proteins (genetics)</term><term>Carrier Proteins (metabolism)</term><term>Cell Wall (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Gene Expression (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Phosphoproteins (genetics)</term><term>Phosphoproteins (metabolism)</term><term>Plant Development (genetics)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Structures (growth & development)</term><term>Plant Structures (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Trees (genetics)</term><term>Trees (metabolism)</term><term>Wood (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Bois (croissance et développement)</term><term>Clonage moléculaire (MeSH)</term><term>Développement des plantes (génétique)</term><term>Expression des gènes (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Phosphoprotéines (génétique)</term><term>Phosphoprotéines (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines de transport (génétique)</term><term>Protéines de transport (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Structures de plante (croissance et développement)</term><term>Structures de plante (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term><term>Phosphoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carrier Proteins</term><term>Phosphoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Bois</term><term>Populus</term><term>Structures de plante</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Development</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Structures</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" 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l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Remorins play vital roles in signal transduction, energy transformation, ion flow and transport in plants. Upregulation of remorins correlates with dehiscence and cell maturation; however, no studies have been performed to elucidate the function of remorins in tree species. In this study, a Populus deltoides (Marsh.) plasma membrane-binding protein remorin gene (PdREM) was cloned and characterized by investigating its expression pattern and creating transgenic hybrid poplar (P. davidiana Dode × P. bolleana Lauche) lines expressing sense or antisense PdREM. PdREM was specifically expressed in leaf buds, and immature and mature phloem in P. deltoides. Downregulation of PdREM increased plant height, stem diameter, number of leaves, size of the xylem and phloem zones and induced expression of cell wall biosynthesis- and microfibril angle (MFA)-related genes. Overexpression of PdREM retarded vegetative growth. PdREM may negatively regulate vascular growth by inhibiting secondary cell wall expansion in poplar. In addition, antisense PdREM transgenic poplar had a lower MFA, suggesting that PdREM might contribute to sheet strength and wood properties in poplar. This study sheds light on the molecular mechanism of PdREM in P. deltoides growth and development, and lays the foundation for future functional genomics research into wood formation and the genetic engineering of forest trees with improved wood quality traits.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24072517</PMID><DateCompleted><Year>2014</Year><Month>06</Month><Day>16</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>10</Issue><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and functional analysis of the Populus deltoides remorin gene PdREM.</ArticleTitle><Pagination><MedlinePgn>1111-21</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpt072</ELocationID><Abstract><AbstractText>Remorins play vital roles in signal transduction, energy transformation, ion flow and transport in plants. Upregulation of remorins correlates with dehiscence and cell maturation; however, no studies have been performed to elucidate the function of remorins in tree species. In this study, a Populus deltoides (Marsh.) plasma membrane-binding protein remorin gene (PdREM) was cloned and characterized by investigating its expression pattern and creating transgenic hybrid poplar (P. davidiana Dode × P. bolleana Lauche) lines expressing sense or antisense PdREM. PdREM was specifically expressed in leaf buds, and immature and mature phloem in P. deltoides. Downregulation of PdREM increased plant height, stem diameter, number of leaves, size of the xylem and phloem zones and induced expression of cell wall biosynthesis- and microfibril angle (MFA)-related genes. Overexpression of PdREM retarded vegetative growth. PdREM may negatively regulate vascular growth by inhibiting secondary cell wall expansion in poplar. In addition, antisense PdREM transgenic poplar had a lower MFA, suggesting that PdREM might contribute to sheet strength and wood properties in poplar. This study sheds light on the molecular mechanism of PdREM in P. deltoides growth and development, and lays the foundation for future functional genomics research into wood formation and the genetic engineering of forest trees with improved wood quality traits.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shaofeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Forestry Experiment Center of North China, Chinese Academy of Forestry, Beijing 100023, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Xiaohua</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Bingyu</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Qinjun</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Zanmin</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Mengzhu</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>09</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002352">Carrier Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010750">Phosphoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C103794">remorin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002352" MajorTopicYN="N">Carrier Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010750" MajorTopicYN="N">Phosphoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D063245" MajorTopicYN="N">Plant Development</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018514" MajorTopicYN="N">Plant Structures</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">PdREM</Keyword><Keyword MajorTopicYN="N">Populus deltoides</Keyword><Keyword MajorTopicYN="N">molecular mechanisms</Keyword><Keyword MajorTopicYN="N">wood formation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24072517</ArticleId><ArticleId IdType="pii">tpt072</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpt072</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Hu, Zanmin" sort="Hu, Zanmin" uniqKey="Hu Z" first="Zanmin" last="Hu">Zanmin Hu</name><name sortKey="Huang, Qinjun" sort="Huang, Qinjun" uniqKey="Huang Q" first="Qinjun" last="Huang">Qinjun Huang</name><name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Shaofeng" sort="Li, Shaofeng" uniqKey="Li S" first="Shaofeng" last="Li">Shaofeng Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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